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From 2012.igem.org

Nisa

PCR of GFP (optimized for B. subtilis) with the recieved primers
T melting Fwd= 57C
T melting Rev = 61C
Therefore, make a gradient from 55C-65C (55, 57, 59,61,63,65)


Ligation primer

idea: ligate mulitple fragments of DNA without T4 ligase, but using the PCR method.


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