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From 2012.igem.org

Revision as of 13:00, 23 July 2012 (view source) Elbrich (Talk | contribs) (Created page with "{{HeaderGroningen2012}}") ← Older edit		Revision as of 13:57, 23 July 2012 (view source) Elbrich (Talk | contribs) Newer edit →
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		+
		+
		+	Nisa
		+
		+	PCR of GFP with the recieved primers
		+	T melting Fwd= 57C
		+	T melting Rev = 61C
		+	Therefore, make a gradient from 55-65C
		+
		+
		+	Ligation primer
		+
		+	idea: ligate mulitple fragments of DNA without T4 ligase, but using PCR

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Revision as of 13:57, 23 July 2012

Nisa

PCR of GFP with the recieved primers T melting Fwd= 57C T melting Rev = 61C Therefore, make a gradient from 55-65C

Ligation primer

idea: ligate mulitple fragments of DNA without T4 ligase, but using PCR