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Team:Calgary/Project/Accomplish
From 2012.igem.org
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<p><b>In terms of <FONT COLOR=#159900>FRED</FONT>, we...</b></p> | <p><b>In terms of <FONT COLOR=#159900>FRED</FONT>, we...</b></p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/3/31/UCalgary2012_FRED_Index_Box.png" style="float: right; padding: | + | <img src="https://static.igem.org/mediawiki/2012/3/31/UCalgary2012_FRED_Index_Box.png" style="float: right; padding: 10px;"></img> |
<ul> | <ul> | ||
| - | <li><p> Constructed a transposon library in <i>Pseudomonas</i>, identifying two positive hits sensitive to a variety of tailings pond toxins.</p></li> | + | <li><p> Constructed a <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/FRED/Detecting#library">transposon library</a> in <i>Pseudomonas</i>, identifying two positive hits sensitive to a variety of tailings pond toxins.</p></li> |
| - | <li><p>Submitted and electrochemically characterized the function of two novel hydrolase enzymes from <i>E. coli</i>, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise.</p></li> | + | <li><p>Submitted and electrochemically characterized the function of <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting#hydrolase">two novel hydrolase enzymes</a> from <i>E. coli</i>, demonstrating the validity and potential of a <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting#output">triple-output system</a> with high sensitivity and little background noise.</p></li> |
| - | <li><p>Designed and wet-lab verified a kinetic model of electrochemical gene expression.</p></li> | + | <li><p>Designed and wet-lab verified a <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Modelling">kinetic model</a> of electrochemical gene expression.</p></li> |
| - | <li><P>Designed both | + | <li><P>Designed both <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Prototype#hardware">hardware</a> and <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Prototype#software">software</a> for a biosensor prototype.</p></li></ul> |
| - | <br><p>In terms of | + | <br> |
| - | <img src="https://static.igem.org/mediawiki/2012/c/c3/UCalgary2012_OSCAR_Index_Box.png" style="float: right; padding: | + | |
| + | <p><b>In terms of <FONT COLOR=#1088CC>OCSAR, we..</FONT></b></p> | ||
| + | <img src="https://static.igem.org/mediawiki/2012/c/c3/UCalgary2012_OSCAR_Index_Box.png" style="float: right; padding: 10px;"></img> | ||
<ul> | <ul> | ||
| - | <li><p>Demonstrated the successful conversion of naphthenic acids into hydrocarbons using Washington 2011's PetroBrick.</p></li> | + | <li><p>Demonstrated the successful conversion of <b>naphthenic acids into hydrocarbons</b> using Washington 2011's PetroBrick.</p></li> |
| - | <li><P>Documented the functionality of an alternative enzyme to the PetroBrick (<i>oleT</i>) in producing alkenes from fatty acids.</p></li> | + | <li><P>Documented the functionality of an <b>alternative enzyme to the PetroBrick</b> (<i>oleT</i>) in producing alkenes from fatty acids.</p></li> |
| - | <li><P>Modified an existing <i>xylE</i> part to show the degradation of catechol into a product, which is then degraded into hydrocarbons using the PetroBrick or OleT enzyme.</p></li> | + | <li><P>Modified an existing <i>xylE</i> part to show the degradation of <b>catechol into a product, which is then degraded into hydrocarbons</b> using the PetroBrick or OleT enzyme.</p></li> |
| - | <li><P>Designed, built, and tested a functioning bioreactor system in which to house our toxin degrading strain.</p></li> | + | <li><P>Designed, built, and tested a <b>functioning bioreactor</b> system in which to house our toxin degrading strain.</p></li> |
| - | <li><P>Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results through modification of growth media.</p></li> | + | <li><P>Used flux variability analysis to <b>optimize the production of our hydrocarbons</b>, surpassing Washington’s previous results through modification of growth media.</p></li> |
| - | <li><P>Demonstrated the successful degradation of carbazole and DBT by our model strains.</p></li> | + | <li><P>Demonstrated the successful <b>degradation of carbazole and DBT</b> by our model strains.</p></li> |
| - | <li><P>Submitted sequenced BioBricks for the removal of nitrogen and sulfur from various compounds and mutagenized eight separate genes to remove illegal cut sites.</p></li> | + | <li><P><b>Submitted sequenced BioBricks</b> for the removal of nitrogen and sulfur from various compounds and <b>mutagenized eight separate genes</b> to remove illegal cut sites.</p></li> |
| - | <li><P>Submitted and characterized a new catalase generator as well as a novel oxido-reductase enzyme for use in our desulfurization project.</p></li> | + | <li><P>Submitted and characterized a <b>new catalase generator</b> as well as a <b>novel oxido-reductase enzyme</b> for use in our desulfurization project.</p></li> |
| - | <li><P>Had an amazing summer and learned a ton!</p></li></ul> | + | <li><P><b>Had an amazing summer and learned a ton!</b></p></li></ul> |
</p> | </p> | ||
Revision as of 05:31, 23 October 2012
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Accomplishments
Our team had many accomplishments over the course of this summer.
In our Human Practices project, we...
Established a dialogue between industry experts in order to inform the design of our project.
Led a discussion through the oil sands leadership initiative (OSLI) on the need and potential for the use of synthetic biology in the oil sands.
Submitted novel riboswitch, promoter and regulatory parts for use in the tight control of killswitch applications and beyond.
Submitted and characterized both a magnesium riboswitch/promoter GFP construct and a magnesium riboswitch/promoter kill gene construct.
Partnered with the UBC iGEM team in order to build and better optimize the dsz desulfurization operon.
Showcased our project to our city and the world through various outreach initiatives including a TEDxCalgary City 2.0 talk.
Premiered and beta-tested our video game LAB ESCAPE! at the Calgary Telus Spark World of Science.
In terms of FRED, we...
Constructed a transposon library in Pseudomonas, identifying two positive hits sensitive to a variety of tailings pond toxins.
Submitted and electrochemically characterized the function of two novel hydrolase enzymes from E. coli, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise.
Designed and wet-lab verified a kinetic model of electrochemical gene expression.
Designed both hardware and software for a biosensor prototype.
In terms of OCSAR, we..
Demonstrated the successful conversion of naphthenic acids into hydrocarbons using Washington 2011's PetroBrick.
Documented the functionality of an alternative enzyme to the PetroBrick (oleT) in producing alkenes from fatty acids.
Modified an existing xylE part to show the degradation of catechol into a product, which is then degraded into hydrocarbons using the PetroBrick or OleT enzyme.
Designed, built, and tested a functioning bioreactor system in which to house our toxin degrading strain.
Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results through modification of growth media.
Demonstrated the successful degradation of carbazole and DBT by our model strains.
Submitted sequenced BioBricks for the removal of nitrogen and sulfur from various compounds and mutagenized eight separate genes to remove illegal cut sites.
Submitted and characterized a new catalase generator as well as a novel oxido-reductase enzyme for use in our desulfurization project.
Had an amazing summer and learned a ton!
