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Team:Calgary/Notebook/Protocols/m9media
From 2012.igem.org
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<li>0.0152 grams CaCl<font style="text-transform: lowercase;">2</font>.2H<font style="text-transform: lowercase;">2</font>O</li> | <li>0.0152 grams CaCl<font style="text-transform: lowercase;">2</font>.2H<font style="text-transform: lowercase;">2</font>O</li> | ||
<li>0.0100 grams Thiamine</li> | <li>0.0100 grams Thiamine</li> | ||
| - | <li>0.007g of FeCl<font style="text-transform: lowercase;">2</font>.4H<font style="text-transform: lowercase;">2</font>O</li> | + | <li>0.007g of FeCl<font style="text-transform: lowercase;">2</font>.4H<font style="text-transform: lowercase;">2</font>O</li></ul> |
| - | </ul> | + | <li>Add the filter sterilized solution into the autoclaved media along with any other additives (e.g. antibiotics). Store at 4°C</li> |
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Revision as of 00:46, 4 October 2012
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M9 Minimal Media Preparation
- Dissolve the following salts in about 500mL of ddH2O:
- 12.8 grams anhydrous Na2HPO4
- 3.0 grams KH2PO4
- 0.5 grams NaCl
- 1.0 gram NH4Cl
- Bring the media to approximately 950 ml with ddH2O and pH to 7.4 with NaOH.
- Autoclave for 20 minutes on slow exhaust. Store at 4°C when finished.
- The following salts were dissolved in 50mL of water and cold filtered with a sterilized 0.22 micron filter:
- 10.8 grams glucose
- 0.19g of MgCl2
- 0.0152 grams CaCl2.2H2O
- 0.0100 grams Thiamine
- 0.007g of FeCl2.4H2O
- Add the filter sterilized solution into the autoclaved media along with any other additives (e.g. antibiotics). Store at 4°C
