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Team:Calgary/Notebook/Protocols/m9media
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<li>Dissolve the following salts in about 500mL of ddH<font style="text-transform: lowercase;">2</font>O: | <li>Dissolve the following salts in about 500mL of ddH<font style="text-transform: lowercase;">2</font>O: | ||
<ul> | <ul> | ||
| - | <li>12.8 grams Na<font style="text-transform: lowercase;">2</font>HPO<font style="text-transform: lowercase;">4</font></li> | + | <li>12.8 grams anhydrous Na<font style="text-transform: lowercase;">2</font>HPO<font style="text-transform: lowercase;">4</font></li> |
<li>3.0 grams KH<font style="text-transform: lowercase;">2</font>PO<font style="text-transform: lowercase;">4</font></li> | <li>3.0 grams KH<font style="text-transform: lowercase;">2</font>PO<font style="text-transform: lowercase;">4</font></li> | ||
<li>0.5 grams NaCl</li> | <li>0.5 grams NaCl</li> | ||
Revision as of 00:44, 4 October 2012
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M9 Minimal Media Preparation
- Dissolve the following salts in about 500mL of ddH2O:
- 12.8 grams anhydrous Na2HPO4
- 3.0 grams KH2PO4
- 0.5 grams NaCl
- 1.0 gram NH4Cl
- Bring the media to approximately 950 ml with ddH2O and pH to 7.4 with NaOH.
- Autoclave for 20 minutes on slow exhaust. Store at 4°C when finished.
- The following salts were dissolved in 50mL of water and cold filtered with a sterilized 0.22 micron filter:
- 10.8 grams glucose
- 0.19g of MgCl2
- 0.0152 grams CaCl2.2H2O
- 0.0100 grams Thiamine
- 0.007g of FeCl2.4H2O
