Team:Calgary/Project/Accomplish
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<li><p>Submitted novel riboswitch, promoter and regulatory elements for use in the tight control of killswitch applications and beyond.</p></li> | <li><p>Submitted novel riboswitch, promoter and regulatory elements for use in the tight control of killswitch applications and beyond.</p></li> | ||
<li><p>Submitted and characterized both a magnesium riboswitch/promoter GFP construct and a magnesium riboswitch/promoter kill gene construct.</p></li> | <li><p>Submitted and characterized both a magnesium riboswitch/promoter GFP construct and a magnesium riboswitch/promoter kill gene construct.</p></li> | ||
- | <li><p>Showcased our project to our city and the world through various outreach initiatives including a | + | <li><p>Showcased our project to our city and the world through various outreach initiatives including a Ted talk.</p></li> |
- | <li><p>Premiered and beta tested a video game at the Calgary Telus | + | <li><p>Premiered and beta-tested a video game at the Calgary Telus Spark World of Science.</p></li></ul> |
<br><p><b>In terms of FRED, we ...</b></p> | <br><p><b>In terms of FRED, we ...</b></p> | ||
<ul> | <ul> | ||
- | <li><p> Constructed a transposon library in | + | <li><p> Constructed a transposon library in <i>Pseudomonas</i>, identifying two positive hits sensitive to a variety of tailings pond toxins.</p></li> |
- | <li><p>Submitted and | + | <li><p>Submitted and electrochemically characterized the function of two novel hydrolase enzymes from <i>E. coli</i>, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise.</p></li> |
<li><p>Designed and wet-lab verified a kinetic model of electrochemical gene expression.</p></li> | <li><p>Designed and wet-lab verified a kinetic model of electrochemical gene expression.</p></li> | ||
<li><P>Designed both software and hardware for a biosensor prototype.</p></li></ul> | <li><P>Designed both software and hardware for a biosensor prototype.</p></li></ul> | ||
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<ul> | <ul> | ||
- | <li><p>Demonstrated the successful conversion of naphthenic acids into hydrocarbons using | + | <li><p>Demonstrated the successful conversion of naphthenic acids into hydrocarbons using Washington 2011's PetroBrick.</p></li> |
- | <li><P>Documented the functionality of an alternative enzyme (oleT) in producing alkenes from fatty acids.</p></li> | + | <li><P>Documented the functionality of an alternative enzyme to the PetroBrick (<i>oleT</i>) in producing alkenes from fatty acids.</p></li> |
- | <li><P>Modified an existing xylE part to show the degradation of catechol into, | + | <li><P>Modified an existing <i>xylE</i> part to show the degradation of catechol into a product, which is then degraded into hydrocarbons using the PetroBrick or OleT enzyme.</p></li> |
- | <li><P>Designed built and tested a functioning bioreactor system in which to house our toxin degrading strain.</p></li> | + | <li><P>Designed, built, and tested a functioning bioreactor system in which to house our toxin degrading strain.</p></li> |
- | <li><P>Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results | + | <li><P>Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results through modification of growth media.</p></li> |
<li><P>Demonstrated the successful degradation of carbazole and DBT by our model strains.</p></li> | <li><P>Demonstrated the successful degradation of carbazole and DBT by our model strains.</p></li> | ||
- | <li><P>Submitted sequenced BioBricks for the removal of nitrogen and | + | <li><P>Submitted sequenced BioBricks for the removal of nitrogen and sulfur from various compounds.</p></li> |
- | <li><P>Submitted and characterized a new catalase | + | <li><P>Submitted and characterized a new catalase generator as well as a novel oxido-reductase enzyme for use in our desulfurization project.</p></li> |
<li><P>Had an amazing summer and learned a ton!</p></li></ul> | <li><P>Had an amazing summer and learned a ton!</p></li></ul> | ||
Revision as of 17:23, 3 October 2012
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Accomplishments
In our Human Practices project, we ...
Established a dialogue between industry experts in order to inform the design of our project.
Led a discussion through the oil sands leadership initiative (OSLI) on the need and potential for the use of synthetic biology in the oil sands.
Submitted novel riboswitch, promoter and regulatory elements for use in the tight control of killswitch applications and beyond.
Submitted and characterized both a magnesium riboswitch/promoter GFP construct and a magnesium riboswitch/promoter kill gene construct.
Showcased our project to our city and the world through various outreach initiatives including a Ted talk.
Premiered and beta-tested a video game at the Calgary Telus Spark World of Science.
In terms of FRED, we ...
Constructed a transposon library in Pseudomonas, identifying two positive hits sensitive to a variety of tailings pond toxins.
Submitted and electrochemically characterized the function of two novel hydrolase enzymes from E. coli, demonstrating the validity and potential of a triple-output system with high sensitivity and little background noise.
Designed and wet-lab verified a kinetic model of electrochemical gene expression.
Designed both software and hardware for a biosensor prototype.
In terms of OCSAR, we ...
Demonstrated the successful conversion of naphthenic acids into hydrocarbons using Washington 2011's PetroBrick.
Documented the functionality of an alternative enzyme to the PetroBrick (oleT) in producing alkenes from fatty acids.
Modified an existing xylE part to show the degradation of catechol into a product, which is then degraded into hydrocarbons using the PetroBrick or OleT enzyme.
Designed, built, and tested a functioning bioreactor system in which to house our toxin degrading strain.
Used flux variability analysis to optimize the production of our hydrocarbons, surpassing Washington’s previous results through modification of growth media.
Demonstrated the successful degradation of carbazole and DBT by our model strains.
Submitted sequenced BioBricks for the removal of nitrogen and sulfur from various compounds.
Submitted and characterized a new catalase generator as well as a novel oxido-reductase enzyme for use in our desulfurization project.
Had an amazing summer and learned a ton!