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Team:Calgary/Notebook/Protocols/mgtacircuit
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Revision as of 16:58, 3 October 2012
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Characterization of the mgtA regulation with S7 killgene
- Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.
- Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.
