Team:Calgary/Notebook/Protocols/mgtacircuit

From 2012.igem.org

(Difference between revisions)
Line 7: Line 7:
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.</li>
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.</li>
<li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li>
<li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li>
-
<br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+
<br><br><br><br><br><br><br><br><br><br><br><br>
</html>}}
</html>}}

Revision as of 03:39, 2 October 2012

Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!

Characterization of mgtA circuit with S7 promoter

  • Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
  • The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
  • Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.
  • Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.












    • Retrieved from "http://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit"