"
Team:Calgary/Notebook/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
<ul> | <ul> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<h2>Electrochemical Techniques</h2> | <h2>Electrochemical Techniques</h2> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process25">Electrode Plating</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process25">Electrode Plating</a></li> | ||
Revision as of 00:42, 2 October 2012
Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!
Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Electrode Plating
- Buffer Preparation
- Electrical Settings
- Testing For Oxidation
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-lacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the lacI-lacZ I732901 Gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
