Team:Calgary/Notebook/Protocols

From 2012.igem.org

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<p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p>
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<ul>
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<h2>Microalgae Protocols</h2>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal Transformation</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process7">Chloroplast Isolation</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process8">Nuclear DNA Extraction from Algae</a></li>
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<h2>Pseudomonas Biotinylation Techniques</h2>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process1">Plasmid Isolation (from <i>Pseudomonas spp.</i>)</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process24">Genomic DNA Isolation (from <i>Pseudomonas spp.</i>)</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process22">Biotinylation/Immunoprecipitation of Naphthenic Acids</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process23">Conjugation of <i>E. coli</i> plasmids to <i>Pseudomonas spp.</i></a></li>
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<h2>Electrochemical Techniques</h2>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process25">Electrode Plating</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process26">Buffer Preparation</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process27">Electrical Settings</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process28">Testing For Oxidation</a></li>
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<h2>General Protocols</h2>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process21">Plasmid Isolation (from <i>E. coli</i>)</a></li>
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        <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process2">RNA Extraction</a></li>
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        <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li>
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        <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li>
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        <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD-<i>lacZ</i> Construct</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process9">Taq PCR Protocol</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process11">Gel Extraction</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process12">Testing the <i>lacI-lacZ</i> I732901 Gene</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process15">Construction Techniques</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process16">Agarose Gel Electrophresis</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process17">LB Agar Plates</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process18">Overnight Cultures</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process19">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li>
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process20">Preparing Glycerol Stocks (<i>E. coli</i>)
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</ul>
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