Team:University College London/LabBook/Week15

From 2012.igem.org

(Difference between revisions)
(15.1)
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- pstA promoter (BBA_I719005) and the GFP (BBA_I712069)</p>
- pstA promoter (BBA_I719005) and the GFP (BBA_I712069)</p>
- the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.</p>
- the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.</p>
 +
[[Image:Gel 1 week 15.jpg]]
<div class="experiment"></div>
<div class="experiment"></div>

Revision as of 17:38, 26 September 2012

15.1

Moday (17.09.12) 3A assembly

Aim – Start 3A assembly to ligate:

- the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct

- the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct

- the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct

- the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct

- pstA promoter (BBA_I719005) and the GFP (BBA_I712069)

- the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.

[[Image:Gel 1 week 15.jpg]]

15.2

15.3