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Team:Groningen/Notebook/Wetwork 21August2012
From 2012.igem.org
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GC-MS: blank runs of the three organic solvents used<br> | GC-MS: blank runs of the three organic solvents used<br> | ||
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| + | Nisa <br> | ||
| + | PCR for checking the construct pigment <br> | ||
| + | result: PCR result was ran in an agarose gel. The result showed multifragment DNA which can imply multi-annealing temperature for every PCR reaction. We cut the DNA fragment with the correct size and purified it. | ||
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
Revision as of 13:54, 26 September 2012
Tom
Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees celcius.
Result: None of the combined promotors with GFP resulted in the correct size band.
Emeraldo
Cut AmilGFP, BBa_K274110, and pSac-Cm+terminator with EcoRI and PstI, run on the gel and excised the correct bands (the AmilGFP band was not detected). Ligation reaction for cut BBa_k274110 and cut pSac-CM+terminator. Transformation of E.coli DH5alpha with the ligated product.
Arjan
GC-MS: blank runs of the three organic solvents used
Nisa
PCR for checking the construct pigment
result: PCR result was ran in an agarose gel. The result showed multifragment DNA which can imply multi-annealing temperature for every PCR reaction. We cut the DNA fragment with the correct size and purified it.
Back to notebook
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