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Team:Groningen/Notebook/Wetwork 2August2012
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| - | Re-did E. coli DH5a transformation (as performed on Sunday) | + | <body><br><br><br> |
| - | + | <p class="margin"> | |
| - | Emeraldo | + | Arjan<br> |
| - | + | <br> | |
| - | Digestion of lycopene (BBa_K274110) and (expected) B. subtilis backbone pSac-Cm using EcoRI and SpeI | + | Did last TAMC measurement (24:00 sample).<br> |
| - | + | <br> | |
| - | Ligated the two fragments together (at 25oC, 2 h). Ligation ratio backbone:insert gene = 1:2 | + | Nisa<br> |
| - | + | <br> | |
| - | Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis. | + | Re-did E. coli DH5a transformation (as performed on Sunday)<br> |
| - | + | <br> | |
| - | < | + | Emeraldo<br> |
| + | <br> | ||
| + | Digestion of lycopene (BBa_K274110) and (expected) B. subtilis backbone pSac-Cm using EcoRI and SpeI<br> | ||
| + | <br> | ||
| + | Ligated the two fragments together (at 25oC, 2 h). Ligation ratio backbone:insert gene = 1:2<br> | ||
| + | <br> | ||
| + | Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis.<br> | ||
| + | <br> | ||
| + | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
| + | <br><br></p></body> | ||
</html> | </html> | ||
{{Template:SponsorsGroningen2012}} | {{Template:SponsorsGroningen2012}} | ||
Latest revision as of 12:52, 26 September 2012
Arjan
Did last TAMC measurement (24:00 sample).
Nisa
Re-did E. coli DH5a transformation (as performed on Sunday)
Emeraldo
Digestion of lycopene (BBa_K274110) and (expected) B. subtilis backbone pSac-Cm using EcoRI and SpeI
Ligated the two fragments together (at 25oC, 2 h). Ligation ratio backbone:insert gene = 1:2
Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis.
Back to notebook
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