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Team:Grenoble/Human Practice/Cost
From 2012.igem.org
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However, if we examine the reliability of the kit (see <a href="https://2012.igem.org/wiki/index.php?title=Team:Grenoble/Modeling/Amplification/Stochastic">modeling stochastic section</a>) it appears that thanks to the designed And Gate in the amplification module, the probability of having a false positive response doesn’t exceed 0.43%. Compared to the reliability of a PCR (93% <a href="https://2012.igem.org/Team:Grenoble/Human_Practice/Cost#ref">[1]</a> ) this value is highly satisfying. | However, if we examine the reliability of the kit (see <a href="https://2012.igem.org/wiki/index.php?title=Team:Grenoble/Modeling/Amplification/Stochastic">modeling stochastic section</a>) it appears that thanks to the designed And Gate in the amplification module, the probability of having a false positive response doesn’t exceed 0.43%. Compared to the reliability of a PCR (93% <a href="https://2012.igem.org/Team:Grenoble/Human_Practice/Cost#ref">[1]</a> ) this value is highly satisfying. | ||
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| + | In order to enhance even more the reliability of our system, we decided to set a second design based on a 96-well plate. Thus, the probability that a false occurs diminishes significantly: If no Golden staph has to be detected, no more than one sample out of 96 gets a visible output signal. | ||
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| + | <center><img src="https://static.igem.org/mediawiki/2012/8/86/Device_gre.PNG"></center> | ||
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| - | [1] Ralf M. Hagen, Irene Seegmüller ,Jila Navai et al. Development of a real-time PCR assay for rapid identification of methicillin-resistant Staphylococcus aureus from clinical samples. International Journal of Medical Microbiology, 2005, 295, 77–86. | + | <b>[1]</b> <a href="https://www.msu.edu/user/msuhla/mod10_3.pdf">Ralf M. Hagen, Irene Seegmüller ,Jila Navai et al. Development of a real-time PCR assay for rapid identification of methicillin-resistant Staphylococcus aureus from clinical samples. International Journal of Medical Microbiology, 2005, 295, 77–86.</a> |
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Revision as of 23:35, 25 September 2012
Design of the device and comparative study of cost assessment
Specifications
Based on the requirements of the medical field (see the section meeting for further details), we set our pathogen detection specifications. So, sEnsiColi should be:- Sensitive
- Reliable (little false positives)
- Fast
- Easy to use
- Lower in price than the current methods.
Design of sEnsiColi
Regarding the specifications above, we set two possible designs for sEnsiColi. The first one is intended for prevention purposes such as assessing the contamination level of hospital’s rooms. The design of this detection-kit is given by the picture below:
