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Team:Groningen/Notebook/Wetwork 5August2012
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Revision as of 13:30, 25 September 2012
Nisa / Emeraldo
Plasmid isolation from E. coli: 1.) 4 colonies alsT (150) promoter + GFP in pSB1C3 2.) 4 colonies alsT (250) promoter + GFP in pSB1C3 3.) 4 colonies alsT (300) promoter + GFP in pSB1C3 4.) 4 colonies alsT (500) promoter + GFP in pSB1C3 5.) 2 colonies of LacI promoter in pSB1C3 6.) pSac-CM + lycopene 7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)
PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.
