Team:Grenoble/Project/Abstract

From 2012.igem.org

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huge impacts on our societies and ways diagnosis and prevention will be performed.  
huge impacts on our societies and ways diagnosis and prevention will be performed.  
In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.  
In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.  
-
It consists of three interconnected modules: 1- Detection, 2- Amplification/Communication and 3- Output.
+
<br/><br/>It consists of three interconnected modules:
 +
<ol>
 +
<li>Detection</li>
 +
<li>Amplification/Communication</li>
 +
<li>Output</li>
 +
</ol>
The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false  
The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false  
positive outputs. Once amplified and filtered, the signal is transmitted to neighboring  
positive outputs. Once amplified and filtered, the signal is transmitted to neighboring  
bacteria via a diffusible molecule. In turn, the amplification loop is triggered which  
bacteria via a diffusible molecule. In turn, the amplification loop is triggered which  
-
leads to the production of a measurable fluorescence output. The design of our  
+
leads to the production of a measurable fluorescence output. <br/>
 +
<br/>The design of our  
network is easily adaptable to different input signals by using other receptor domains.  
network is easily adaptable to different input signals by using other receptor domains.  
<center> <h4>Scheme of the concept</h4> </center>
<center> <h4>Scheme of the concept</h4> </center>

Revision as of 00:58, 25 September 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

sEnsiColi: A tunable and reliable ultra-sensitive detector

Multi-resistant bacteria are a worldwide issue which in a very near future will have huge impacts on our societies and ways diagnosis and prevention will be performed. In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.

It consists of three interconnected modules:
  1. Detection
  2. Amplification/Communication
  3. Output
The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false positive outputs. Once amplified and filtered, the signal is transmitted to neighboring bacteria via a diffusible molecule. In turn, the amplification loop is triggered which leads to the production of a measurable fluorescence output.

The design of our network is easily adaptable to different input signals by using other receptor domains.

Scheme of the concept

Scheme of the concept

Theoretical response of the device depending on the input signal


Theoretical response of the device depending on the input signal

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