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Team:Grenoble/Biology/Network
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<a href="https://2012.igem.org/Team:Grenoble/Biology/Network#20" class="schema" ><img src="https://static.igem.org/mediawiki/2012/b/bb/Circuit2.png" alt="" style="position: relative; top: 54px;" /></a> | <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#20" class="schema" ><img src="https://static.igem.org/mediawiki/2012/b/bb/Circuit2.png" alt="" style="position: relative; top: 54px;" /></a> | ||
<a href="https://2012.igem.org/Team:Grenoble/Biology/Network#10" class="schema" ><img src="https://static.igem.org/mediawiki/2012/a/a4/Circuit_gre.png" alt="" style="position: relative; top: -468px;"/></a> | <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#10" class="schema" ><img src="https://static.igem.org/mediawiki/2012/a/a4/Circuit_gre.png" alt="" style="position: relative; top: -468px;"/></a> | ||
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<h2 id="10">Signaling module</h2> | <h2 id="10">Signaling module</h2> | ||
Revision as of 22:27, 23 September 2012
Network details
Our system is divided in two modules:- signaling module
- amplification module
Signaling module
The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.
Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.
The dipeptide binds to his receptor which is an engineered receptor:
- the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
- the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation
Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator.
Amplification module
The amplification module allows our bacterial strain to amplify the input signal and to produce an output signal = fluorescence.
Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.
Adenyl cyclase is an enzyme which catalyse the conversion of ATP (Adenosine Tri-Phosphate) to cAMP (cyclic Adenosine Mono-Phosphate).
cAMP binds to CRP (C-reactive protein) and then this complex allows the production of AraC by activating pMalT promoter.
In the presence of arabinose, AraC, with cAMP-CRP, activates the pAraBAD promoter which allow the production of:
- adenyl cyclase which reproduce cAMP forming an amplification loop
- GFP (Green Fluorescent Protein) = our output signal
