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Team:Grenoble/Biology/Network
From 2012.igem.org
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| - | Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator. | + | Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator. |
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<h2 id="2">Amplification module</h2> | <h2 id="2">Amplification module</h2> | ||
| + | The amplification module allows out bacterial strain to amplify the input signal and to produce an output signal = fluorescence.<br/> | ||
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| + | Once OmpR is phosphorylated, it can allows the production of adenyl cyclase by activate the OmpC promotor. | ||
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Revision as of 21:35, 23 September 2012
Network details
Signaling module
The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.
The dipeptide bind to his receptor which is an engineered receptor:
- the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
- the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation
Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator.
Amplification module
The amplification module allows out bacterial strain to amplify the input signal and to produce an output signal = fluorescence.Once OmpR is phosphorylated, it can allows the production of adenyl cyclase by activate the OmpC promotor.
