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Team:Groningen/Notebook/Wetwork 2August2012
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Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis. | Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis. | ||
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Revision as of 19:01, 16 September 2012
Arjan
Did last TAMC measurement (24:00 sample).
Nisa
Re-did E. coli DH5a transformation (as performed on Sunday)
Emeraldo
Digestion of lycopene (BBa_K274110) and (expected) B. subtilis backbone pSac-Cm using EcoRI and SpeI
Ligated the two fragments together (at 25oC, 2 h). Ligation ratio backbone:insert gene = 1:2
Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis.
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