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Team:Groningen/Notebook/Wetwork 16August2012
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Tomvanlente (Talk | contribs) (Created page with "{{HeaderGroningen2012}} Tom Ligation of Psb1c3 with Fnr promotor combined to GFP. For the digestion of Psb1c3, 258.2 ng was used. For the digestion of Fnr promotor and GFP, 239...") |
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Result: After transformation and plasmid isolation all the colonies contained empty plasmids. | Result: After transformation and plasmid isolation all the colonies contained empty plasmids. | ||
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| + | The plates were thrashed in the waste bin (accidentally). Re-do the activities from 14-08-2012 | ||
Revision as of 13:59, 25 September 2012
Tom
Ligation of Psb1c3 with Fnr promotor combined to GFP. For the digestion of Psb1c3, 258.2 ng was used. For the digestion of Fnr promotor and GFP, 239 ng was used. Ligation was done with 25.82 ng Psb1c3 and 36 ng of Fnr promotor + GFP.
Result: After transformation and plasmid isolation all the colonies contained empty plasmids.
Emeraldo
The plates were thrashed in the waste bin (accidentally). Re-do the activities from 14-08-2012
