Gel electrophoresis

Goal

Prepare the gel

1. Add 1 g of agarose powder to 50 mL of 1X TAE buffer.
2. Heat the solution using the microwave until the powder is completely dissolved.
3. Once the solution is cool enough to be touched, pour it into gel cast (don't forget the comb).

Load the gel

1. When the gel is solid, remove carefully the comb.
2. Place the gel in the buffer tank and cover it with the 1X TAE buffer solution.
3. Load each sample into the wells (don't forget the loading dye and the DNA ladder).

Run the gel

1. Connect the generator.
2. Chose the right voltage.
3. Put the device on.