Team:Grenoble/Biology/Protocols/Gel

From 2012.igem.org

(Difference between revisions)
Line 15: Line 15:
         <li>Add 1 g of agarose powder to 50 mL of 1X TAE buffer.</li>
         <li>Add 1 g of agarose powder to 50 mL of 1X TAE buffer.</li>
         <li>Heat the solution using the microwave until the powder is completely dissolved.</li>
         <li>Heat the solution using the microwave until the powder is completely dissolved.</li>
-
         <li>Once the solution is cool enough to be touched, pour it into gel cast (don't forget to pull the comb).</li>
+
         <li>Once the solution is cool enough to be touched, pour it into gel cast (don't forget the comb).</li>
-
       
+
</section>
</section>
 +
 +
<section>
 +
    <h2>Load the gel</h2>
 +
    <ol>
 +
        <li>When the gel is solid, remove carefully the comb.</li>
 +
        <li>Place the gel in the buffer tank and cover it with the buffer solution.</li>
 +
        <li>Load each sample into the wells (don't forget the loading dye and the DNA ladder).</li>
 +
    </ol>
 +
</section>
 +
 +
<section>
 +
    <h2>Run the gel</h2>
 +
    <ol>
 +
        <li>
</div>
</div>

Revision as of 08:56, 28 August 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

Gel electrophoresis

Goal

Separate DNA strands of different lengths.

Prepare the gel

  1. Add 1 g of agarose powder to 50 mL of 1X TAE buffer.
  2. Heat the solution using the microwave until the powder is completely dissolved.
  3. Once the solution is cool enough to be touched, pour it into gel cast (don't forget the comb).

Load the gel

  1. When the gel is solid, remove carefully the comb.
  2. Place the gel in the buffer tank and cover it with the buffer solution.
  3. Load each sample into the wells (don't forget the loading dye and the DNA ladder).

Run the gel

Retrieved from "http://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel"