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Team:Grenoble/Biology/Protocols/Glycerol
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<li>mix 200 L of glycerol and 800 L of liquid culture</li> | <li>mix 200 L of glycerol and 800 L of liquid culture</li> | ||
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Then store the glycerol stock at -80°C. | Then store the glycerol stock at -80°C. | ||
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Revision as of 10:53, 23 August 2012
Glycerol stock preparation
Goal
Prepare glycerol stock is a way to store bacteria during a long period.Protocol
You need to:- grow a liquid culture overnight
- prepare sterile 80% glycerol
- mix 200 L of glycerol and 800 L of liquid culture
Then store the glycerol stock at -80°C.
