Team:Grenoble/Project/Abstract

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<h1>Project Description</h1>
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<h1>sEnsiColi: A tunable and reliable ultra-sensitive detector</h1>
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The Grenoble iGEM team intends to build an ultra sensitive detector. The goal is to produce a detector which can greatly amplify an extremely low input signal. It consists of three modules:<br/>
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Multi-resistant bacteria are a worldwide issue which in a very near future will have
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huge impacts on our societies and ways diagnosis and prevention will be performed.
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    <li>the first gets the signal</li>
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In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.  
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    <li>the second amplifies it</li>
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It consists of three interconnected modules: 1- Detection, 2- Amplification/Communication and 3- Output.
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    <li>the third sends the output</li>
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The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false  
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positive outputs. Once amplified and filtered, the signal is transmitted to neighboring  
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bacteria via a diffusible molecule. In turn, the amplification loop is triggered which
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The detection module is a membrane receptor that once activated by a biomolecule actuates an amplification loop. The loop enhances the cell response efficiency. <br/>
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leads to the production of a measurable fluorescence output. The design of our
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The amplification system contains a genetic feed forward loop. It acts as a biologic loud filter aiming at reducing false positive outputs. Once amplified and filtered the signal is transmitted to the neighboring bacteria. In this way a noticeable fluorescent emission is generated. <br/>
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network is easily adaptable to different input signals by using other receptor domains.
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<center> <h4>Scheme of the concept</h4> </center>
<center> <h4>Scheme of the concept</h4> </center>
<center><img src="https://static.igem.org/mediawiki/2012/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/></center>
<center><img src="https://static.igem.org/mediawiki/2012/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/></center>

Revision as of 20:49, 24 September 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

sEnsiColi: A tunable and reliable ultra-sensitive detector

Multi-resistant bacteria are a worldwide issue which in a very near future will have huge impacts on our societies and ways diagnosis and prevention will be performed. In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector. It consists of three interconnected modules: 1- Detection, 2- Amplification/Communication and 3- Output. The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false positive outputs. Once amplified and filtered, the signal is transmitted to neighboring bacteria via a diffusible molecule. In turn, the amplification loop is triggered which leads to the production of a measurable fluorescence output. The design of our network is easily adaptable to different input signals by using other receptor domains.

Scheme of the concept

Scheme of the concept

Theoretical response of the device depending on the input signal


Theoretical response of the device depending on the input signal

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