Team:Groningen/Notebook/Wetwork 25June2012

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
-
Tom
+
<html>
 +
<head>
 +
<style type="text/css">
 +
p.margin{
 +
font-size:14pt;
 +
line-height:14pt;
 +
color:white;
 +
margin-top:0px;
 +
margin-bottom:20px;
 +
margin-left:100px;
 +
margin-right:100px;
 +
}
 +
</style>
 +
</head>
-
Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth.
+
<body>
 +
<p class="margin">
 +
Tom <br>
-
Nisa and Emeraldo
+
Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth. <br><br>
-
Preparation for another B. subtilis transformation (BBa_K090403+pigment):
+
Nisa and Emeraldo <br>
-
1.) Preparation of Transformation medium (SMM)
+
-
2.) O/N culture of B. subtilis in SMM
+
-
3.) O/N culture E. coli with BBa_K090403
+
-
Primer design for P alsT with addition of prefix and suffix
+
Preparation for another B. subtilis transformation (BBa_K090403+pigment):<br>
-
1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene)
+
1.) Preparation of Transformation medium (SMM)<br>
-
2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes
+
2.) O/N culture of B. subtilis in SMM<br>
-
3.) Order the primers
+
3.) O/N culture E. coli with BBa_K090403<br>
 +
<br>
 +
Primer design for P alsT with addition of prefix and suffix<br>
 +
1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene)<br>
 +
2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes<br>
 +
3.) Order the primers<br>
 +
<br>
 +
Preparing starch agar and iodine for checking purpose of B. subtilis transformant <br>
 +
(B. subtilis transformants of BBa_K090403 should not be able to degrade starch)<br>
-
Preparing starch agar and iodine for checking purpose of B. subtilis transformant
+
<br>
-
(B. subtilis transformants of BBa_K090403 should not be able to degrade starch)
+
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
 +
</p>
 +
</body>
 +
</html>
 +
 
 +
{{Template:SponsorsGroningen2012}}

Revision as of 15:48, 15 September 2012




Tom
Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth.

Nisa and Emeraldo
Preparation for another B. subtilis transformation (BBa_K090403+pigment):
1.) Preparation of Transformation medium (SMM)
2.) O/N culture of B. subtilis in SMM
3.) O/N culture E. coli with BBa_K090403

Primer design for P alsT with addition of prefix and suffix
1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene)
2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes
3.) Order the primers

Preparing starch agar and iodine for checking purpose of B. subtilis transformant
(B. subtilis transformants of BBa_K090403 should not be able to degrade starch)

Back to notebook

Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Notebook/Wetwork_25June2012"