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Team:Calgary/Notebook/Protocols/PetroBrick Validation Assay
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<P> Following extraction of the samples and controls for Petrobrick experiments, samples were concentrated to 0.3 mL and placed into autosampler vials. via autoinjection on an Agilent GC-MS system (model 7890A GC, model 5975C inert XL MSD) equipped with an HP-1 capillary column (50 m x 0.32 mm i.d. x 0.2 μm film, Agilent Technologies, Inc.). The samples were analyzed in splitless mode, with the inlet held at 270°C. The oven was initially held at 100°C for 5 min, then increased at a rate of 8°C/min to a final temperature of 300°C that was held for 5 min. </p> | <P> Following extraction of the samples and controls for Petrobrick experiments, samples were concentrated to 0.3 mL and placed into autosampler vials. via autoinjection on an Agilent GC-MS system (model 7890A GC, model 5975C inert XL MSD) equipped with an HP-1 capillary column (50 m x 0.32 mm i.d. x 0.2 μm film, Agilent Technologies, Inc.). The samples were analyzed in splitless mode, with the inlet held at 270°C. The oven was initially held at 100°C for 5 min, then increased at a rate of 8°C/min to a final temperature of 300°C that was held for 5 min. </p> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oextraction">Organic Extraction</a></li> | ||
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Revision as of 22:41, 3 October 2012
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PetroBrick Validation Assay
Following extraction of the samples and controls for Petrobrick experiments, samples were concentrated to 0.3 mL and placed into autosampler vials. via autoinjection on an Agilent GC-MS system (model 7890A GC, model 5975C inert XL MSD) equipped with an HP-1 capillary column (50 m x 0.32 mm i.d. x 0.2 μm film, Agilent Technologies, Inc.). The samples were analyzed in splitless mode, with the inlet held at 270°C. The oven was initially held at 100°C for 5 min, then increased at a rate of 8°C/min to a final temperature of 300°C that was held for 5 min.
