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Team:Calgary/Notebook/Protocols/bactest
From 2012.igem.org
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| + | <li>Mix a solution of water and 5mL <i>E. coli</i> into 5 separate beakers.</li> | ||
| + | <li>Dip each belt into a separate beaker of the previous solution for 3 seconds and remove.</li> | ||
| + | <li>Scrape removed solution onto separate plates, and let bacteria grow overnight in an incubator.</li> | ||
| + | <li>Count bacterial colonies next day of each plate if possible. Judge which belt picked up most bacteria based on plate observation and rank from greatest to worst for inability to pick up bacteria (highest rank indicating least amount of bacteria picked up, i.e. less colonies observed).</li> | ||
| + | </ol> | ||
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Revision as of 07:32, 3 October 2012
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Bacteria Test
- Mix a solution of water and 5mL E. coli into 5 separate beakers.
- Dip each belt into a separate beaker of the previous solution for 3 seconds and remove.
- Scrape removed solution onto separate plates, and let bacteria grow overnight in an incubator.
- Count bacterial colonies next day of each plate if possible. Judge which belt picked up most bacteria based on plate observation and rank from greatest to worst for inability to pick up bacteria (highest rank indicating least amount of bacteria picked up, i.e. less colonies observed).
