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Team:Calgary/Notebook/Protocols/bacgrowth
From 2012.igem.org
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| + | <p>Autoclave the spinner flask with copper sparger in the correct position.</p> | ||
| + | <p>Mix and autoclave 1L LB solution and add to the spinner flask.</p> | ||
| + | <p>Add 1 ml ampicillin and 1ml ampicillin-resistant <i>E coli</i> into the LB media.</p> | ||
| + | <p>Put the spinner flask in the incubator at 37°C.</p> | ||
| + | <p>Remove 200µL for a sample and measure the optical density in reference to the optical density of a LB sample (negative control).</p> | ||
| + | <p>Repeat the experiment three times: one with the spinner only, one with the air sparger only, and one with both air sparger and spinner on.</p> | ||
</html>}} | </html>}} | ||
Revision as of 07:22, 3 October 2012
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Bacterial Growth Experiment
Autoclave the spinner flask with copper sparger in the correct position.
Mix and autoclave 1L LB solution and add to the spinner flask.
Add 1 ml ampicillin and 1ml ampicillin-resistant E coli into the LB media.
Put the spinner flask in the incubator at 37°C.
Remove 200µL for a sample and measure the optical density in reference to the optical density of a LB sample (negative control).
Repeat the experiment three times: one with the spinner only, one with the air sparger only, and one with both air sparger and spinner on.
