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Team:Calgary/Notebook/Protocols/catalase
From 2012.igem.org
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<li>Grow cultures of <i>E. coli J04500-katG+LAA</i> and <i>J04500</i> overnight at 37*C in LB with appropriate antibiotics.</li> | <li>Grow cultures of <i>E. coli J04500-katG+LAA</i> and <i>J04500</i> overnight at 37*C in LB with appropriate antibiotics.</li> | ||
<li>The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.</li> | <li>The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.</li> | ||
| - | <li>The following morning, observe culture turbidity.</li> | + | <li>The following morning, observe and record culture turbidity.</li> |
</ol> | </ol> | ||
Revision as of 02:14, 2 October 2012
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Catalase Assay
- Grow cultures of E. coli J04500-katG+LAA and J04500 overnight at 37*C in LB with appropriate antibiotics.
- The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.
- The following morning, observe and record culture turbidity.
