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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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<h2>General Protocols</h2> | <h2>General Protocols</h2> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/agarosegel">Agarose Gel Electrophresis</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/agarosegel">Agarose Gel Electrophresis</a></li> | ||
| + | <li><a href="">Bacterial Genomic DNA Purification</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li> | ||
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li> | ||
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| - | |||
<li><a href="">Site-Directed Mutagenesis</a></li> | <li><a href="">Site-Directed Mutagenesis</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/taqpcr">Taq PCR Protocol</a></li> | ||
<h2>Electrochemistry Protocols</h2> | <h2>Electrochemistry Protocols</h2> | ||
Revision as of 02:02, 2 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- Gel Extraction
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Site-Directed Mutagenesis
- Taq PCR Protocol
Electrochemistry Protocols
- Electorchemistry protocols (include agarose bead making)
Desulfurization
- Desulphuirzation assay protocol
- Catalase/ hpac assays
Decarboxylation
- Petrobrick/oleT assay protocols/ GC MS
Denitrogenation
Decatecholization
- Decateccholzation (xylE and incubation with the Petrobrick)
Transposon
Kill Switch
- Rhamnose, magnesium and manganese, kill gene assay (nuclease)
Bioreactor
- Bioreactor assays
Modeling
- Modelling validation experiments
