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Team:Groningen/Notebook/Wetwork 25June2012
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Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth. | Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth. | ||
| + | |||
| + | Nisa and Emeraldo | ||
| + | |||
| + | Preparation for another B. subtilis transformation (BBa_K090403+pigment): | ||
| + | 1.) Preparation of Transformation medium (SMM) | ||
| + | 2.) O/N culture of B. subtilis in SMM | ||
| + | 3.) O/N culture E. coli with BBa_K090403 | ||
| + | |||
| + | Primer design for P alsT with addition of prefix and suffix | ||
| + | 1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene) | ||
| + | 2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes | ||
| + | 3.) Order the primers | ||
| + | |||
| + | Preparing starch agar and iodine for checking purpose of B. subtilis transformant | ||
| + | (B. subtilis transformants of BBa_K090403 should not be able to degrade starch) | ||
Revision as of 16:49, 8 July 2012
Tom
Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth.
Nisa and Emeraldo
Preparation for another B. subtilis transformation (BBa_K090403+pigment): 1.) Preparation of Transformation medium (SMM) 2.) O/N culture of B. subtilis in SMM 3.) O/N culture E. coli with BBa_K090403
Primer design for P alsT with addition of prefix and suffix 1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene) 2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes 3.) Order the primers
Preparing starch agar and iodine for checking purpose of B. subtilis transformant (B. subtilis transformants of BBa_K090403 should not be able to degrade starch)
