"
Team:NYMU-Taipei/ymip2.html
From 2012.igem.org
(Difference between revisions)
(Created page with "<html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>NYMU iGEM</title> <link href="http://www.royals.co...") |
|||
| Line 133: | Line 133: | ||
<li><a title="Safety" | <li><a title="Safety" | ||
href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li> | href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li> | ||
| - | <li><a title="Collaboration" | + | <li><a title="Collaboration with NTU" href="https://2012.igem.org/Team:NYMU-Taipei/ymico1.html">Collaboration with NTU</a></li> |
| - | href="https://2012.igem.org/Team:NYMU-Taipei/ | + | <li><a title="Collaboration with Entrepreneur" href="https://2012.igem.org/Team:NYMU-Taipei/ymico2.html">Collaboration with<br /> |
| + | Entrepreneur</a></li> | ||
<li><a title="Parts" | <li><a title="Parts" | ||
href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li> | href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li> | ||
Revision as of 01:17, 27 September 2012
Protocol
Mesenchymal Stem Cells Culturing
- Mesenchymal stem cells from human umbilical cord were provided by Prof. Chun-Min Lo from National Yang Ming University.
- View cultures using a microscopy and after the cultures was full of the dish, remove the spent medium.
- Wash the cell monolayer with PBS and remove it.
- Add HyQTase(1ml per 25 cm2 of surface area) and incubate for 1 minutes.
- After making sure cells were detached, resuspend the cells with medium(more than 5 folds volume of HyQTase).
- Centrifuge under 2000 rpm, 5minutes, 25°C.
- Remove the supernatant and add 5 ml medium to suspend cells.
- Count the cells by Bright Line Counting Chamber. Subject the cell diversity to 104/ml
- Seed the cells for 104/well, 1ml/well in the 24-well plate.
- Until cells grow full of each well, do the coculturing experiment.
