Team:NYMU-Taipei/ymiparts.html

Data for Our Favourite New Parts

1. Main Page - SQR(sulfide quinone reductase), BBa_K896000: Sulfide-dependent anoxygenic photosynthesis, driven by photosystem I (PS/I) alone, among cyanobacteria was first described for Oscillatoria limnetica from Solar Lake. Later photosynthetic sulfide oxidation in O. limnetica led to the discovery of sulfide-quinone reductase (SQR; E.C.1.8.5.), a novel enzyme that transfers electrons from sulfide into the quinone pool.
   
   
2. Main Page - CysI (surfite reductase), BBa_K896001: We have successfully expressed and photoconverted this protein in E. coli cells. It reduces (HSO3)- to H2S using the embedded electron transfer system in E.coli
   
   
3. Main Page - DsrI-DsrII(surfite reductase), BBa_K896002: There are two sulfite reductase operon, DsrI and DsrII, inside Desulfovibrio desulfuricans. They might be membrane form Dsr operon and free form Dsr operon. The Dsr gene’s construction in E.coli, cyanobacteria and used bacteria to remove SO2 in our environment has been accomplished by us.
   
   

Parts We Have Improved

1. Experience - Part: BBa_K896012: When engineered with invasin(inv) from Yersinia pestis and listeriolysin O(llo) from Listeria monocytogenes, new S. elongatus was able to invade into cultured mammalian cells and was capable of symbiosis with eukaryotic cells. The symbiosis possibility of induced pluripotent stem cells from mice and J774 mouse macrophage cell line were evaluated. We had also accomplished the symbiosis experiment.
   
   

We've Also Created and Characterised the Following Parts

1. Main Page - Sulfide quinone reductase(SQR) on neutral site 2 vector, BBa_K896003: SQR could transfer electrons from sulfide into the quinone pool and trigger the sulfide-dependent photosynthesis, which is only based on photosystem I and shuttle the photosystem II down. The neutral site 2 vector(4538 bp) consists of trc promoter(1718-1963 bp), multiple cloning site(1965-2014 bp), and Streptomycin resistence gene(2555-3343 bp).
   
   
2. Main Page – NosZ+NorCB, BBa_K896004: NorCB is composed of two sub-unit – nitric oxide reductase subunit C and B. In fact, these two subunits are adjacent genes. And thus we cloned them together to produce a functional reductase. And NosZ is the structure gene of nitrous reductase. Furthermore, it performs the last procedure of bio-denitrification in our model. Obviously, the reason we cloned NorCB and NosZ on the same plasmid is to connect the continuous denitrifying procedures together.( We clone NosZ and NorCB and ligate them together. However, since there are PstI enzyme cutting site inside the NorCB, so we clone a new pSB1C3 backbone which contain EcoRI.XbaI.SpeI.SbfI enzyme cutting site.)
   
   
3. Main Page - NorCB, BBa_K896005: NorCB(Nitric oxide reductase subunit C and B)can transfer nitric oxide into nitrous oxide. We receive this part from the genomic DNA of Pseudomonas aeruginosa PAO1.
   
   
4. Main Page - NirN-NirS (Nitrite->Nitric oxide reductase), BBa_K896006: Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein
   
   
5. Main Page - Nap( Nitrate->Nitrite reductase), BBa_K896007: Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. Even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor.
   
   
6. Main Page - pYodA (Cd2+ sensing promoter), BBa_K896008: ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. The activity of this promoter is specific affect by cadmium ion and won’t be induced by other ions like zinc, copper, cobalt, and nickel.
   
   
7. Main Page - pYodA+GFP( Cd2+ biosensor ), BBa_K896009: Combine with GFP to prove the expression of pYodA(BBa_K896008).
   
   
8. Main Page - smtA ( Cd2+ absorbing protein), BBa_K896010: Metallothionein (MT) is a family of cysteine-rich, low molecular weight (MW ranging from 500 to 14000 Da) proteins. MTs have the capacity to bind both physiological (such as zinc, copper, selenium) and xenobiotic (such as cadmium, mercury, silver, arsenic) heavy metals through the thiol group of its cysteine residues .
   
   
9. Main Page - MntA (Cd2+ resistent molecule), BBa_K896011