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Team:Grenoble/Biology/AND gate
From 2012.igem.org
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<section> | <section> | ||
| + | <h2>paraBAD</h2> | ||
In order to develop our device we needed a biological AND gate. | In order to develop our device we needed a biological AND gate. | ||
We found a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. | We found a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. | ||
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<section> | <section> | ||
| - | cAMP </ | + | <h2>cAMP</h2> |
In order to make our AND work we need to produce cAMP. | In order to make our AND work we need to produce cAMP. | ||
cAMP is produce by adenyl cyclase (encoded by the cyaA gene). | cAMP is produce by adenyl cyclase (encoded by the cyaA gene). | ||
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<a href="https://2012.igem.org/Team:Grenoble/Biology/AND_gate#1">[1]</a> and binds to the cAMP receptor protein (CRP). | <a href="https://2012.igem.org/Team:Grenoble/Biology/AND_gate#1">[1]</a> and binds to the cAMP receptor protein (CRP). | ||
The corresponding complex (CRP-cAMP) is a transcriptional factor controlling the expression | The corresponding complex (CRP-cAMP) is a transcriptional factor controlling the expression | ||
| - | of more than 220 operons | + | of more than 220 operons <a href="https://2012.igem.org/Team:Grenoble/Biology/AND_gate#1">[2]</a>. |
It has been known for a long time that E. coli actively exports cAMP | It has been known for a long time that E. coli actively exports cAMP | ||
| - | into the growth medium | + | into the growth medium [3]. |
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| - | + | </section> | |
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of nutrients. Nat Rev Microbiol, 6(8), 613–24.</a></li> | of nutrients. Nat Rev Microbiol, 6(8), 613–24.</a></li> | ||
<br/> | <br/> | ||
| - | + | <li><b>[2]</b> <a ref="http://www.ncbi.nlm.nih.gov/pubmed/18974181"target="_blank">Keseler, I. M., Bonavides-Martinez, C., Collado-Vides, J., Gama-Castro, S., Gunsalus, R. P., Johnson, D. A., Krummenacker, M., Nolan, L. M., Paley, S., Paulsen, I. T., Peralta-Gil, M., Santos-Zavaleta, A., Shearer, A. G., & Karp, P. D. 2009. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res, 37(Database issue), D464–70.</a> | |
| + | </br> | ||
| + | <li><b>[3]</b> <a ref="" target="_blank">.......</a></li> | ||
Revision as of 20:50, 26 September 2012
paraBAD
In order to develop our device we needed a biological AND gate. We found a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. The paraBAD promoter has two states; in absence of L-arabinose the paraBAD promoter is repressed by AraC, whereas the the paraC promoter is activated (unless an excess of AraC is present)
cAMP
In order to make our AND work we need to produce cAMP. cAMP is produce by adenyl cyclase (encoded by the cyaA gene). It is an enzyme which catalyses the conversion of ATP to 3’,5’-cAMP. In Escherichia coli, cAMP is involved in carbon catabolite repression [1] and binds to the cAMP receptor protein (CRP). The corresponding complex (CRP-cAMP) is a transcriptional factor controlling the expression of more than 220 operons [2]. It has been known for a long time that E. coli actively exports cAMP into the growth medium [3].References
- [1] Gorke, B., & Stulke, J. 2008. Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol, 6(8), 613–24.
- [2] Keseler, I. M., Bonavides-Martinez, C., Collado-Vides, J., Gama-Castro, S., Gunsalus, R. P., Johnson, D. A., Krummenacker, M., Nolan, L. M., Paley, S., Paulsen, I. T., Peralta-Gil, M., Santos-Zavaleta, A., Shearer, A. G., & Karp, P. D. 2009. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res, 37(Database issue), D464–70.
- [3] .......
