"
Team:University College London/LabBook/Week15
From 2012.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
<div class="experimentContent"></html> | <div class="experimentContent"></html> | ||
== 15.1 == | == 15.1 == | ||
| - | + | Moday (17.09.12) 3A assembly | |
| - | + | ||
| - | <b>Aim – Start 3A assembly to ligate: </b | + | |
| - | + | <b>Aim – Start 3A assembly to ligate: </b> | |
| + | * the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p> | ||
- the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p> | - the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p> | ||
- the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct</p> | - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct</p> | ||
| Line 15: | Line 16: | ||
- the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.</p> | - the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.</p> | ||
[[Image:Gel 1 week 15.jpg]] | [[Image:Gel 1 week 15.jpg]] | ||
| - | + | <html></div> | |
<div class="experiment"></div> | <div class="experiment"></div> | ||
<img id="15.2" src="https://static.igem.org/mediawiki/2012/2/22/Ucl2012-labbook-graph7-2.png" /><div class="experimentContent"> | <img id="15.2" src="https://static.igem.org/mediawiki/2012/2/22/Ucl2012-labbook-graph7-2.png" /><div class="experimentContent"> | ||
Revision as of 17:40, 26 September 2012
15.1
Moday (17.09.12) 3A assembly
Aim – Start 3A assembly to ligate:
- the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p>
- the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p> - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct</p> - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct</p> - pstA promoter (BBA_I719005) and the GFP (BBA_I712069)</p> - the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.</p> File:Gel 1 week 15.jpg
15.2
