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Team:Groningen/Notebook/Wetwork 17August2012
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| - | Tom | + | Tom <br> |
| - | + | <br> | |
| - | PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP. | + | PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP. <br> |
| - | + | <br> | |
| - | Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not | + | Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not appropriate working taq polymerase. <br> |
| - | + | <br> | |
| - | + | <br> | |
| - | Emeraldo | + | Emeraldo <br> |
| - | + | <br> | |
| - | Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product. | + | Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product. <br> |
| + | <br> | ||
| + | <br> | ||
| + | Nisa<br> | ||
| + | Plasmid isolation of the transformans | ||
<html> | <html> | ||
Revision as of 13:31, 26 September 2012
Tom
PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP.
Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not appropriate working taq polymerase.
Emeraldo
Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product.
Nisa
Plasmid isolation of the transformans
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