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Team:Grenoble/Biology/Network
From 2012.igem.org
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<h2 id="2">Amplification module</h2> | <h2 id="2">Amplification module</h2> | ||
| - | The amplification module allows | + | The amplification module allows our bacterial strain to amplify the input signal and to produce an output signal = fluorescence.<br/> |
<br/> | <br/> | ||
Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.<br/> | Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.<br/> | ||
Revision as of 21:43, 23 September 2012
Network details
Our system is divided in two modules:- signaling module
- amplification module
Signaling module
The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.
The dipeptide bind to his receptor which is an engineered receptor:
- the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
- the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation
Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator.
Amplification module
The amplification module allows our bacterial strain to amplify the input signal and to produce an output signal = fluorescence.Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.
Adenyl cyclase is an enzyme which catalyse the conversion of ATP (Adenosine Tri-Phosphate) to cAMP (cyclic Adenosine Mono-Phosphate)
