Revision as of 21:41, 23 September 2012

iGEM Grenoble 2012

iGEM 2012
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Network details

Our system is divided in two modules:

signaling module
amplification module

Signaling module

The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.

Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.

The dipeptide bind to his receptor which is an engineered receptor:

the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation

Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator.

Amplification module

The amplification module allows out bacterial strain to amplify the input signal and to produce an output signal = fluorescence.

Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.
Adenyl cyclase is an enzyme which catalyse the conversion of ATP (Adenosine Tri-Phosphate) to cAMP (cyclic Adenosine Mono-Phosphate)

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 <section>
 <h1>Network details</h1>
+Our system is divided in two modules:
+<ul><li>signaling module
+<li>amplification module
 </section>
 <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#1" class="schema" ><img src="https://static.igem.org/mediawiki/2012/b/bb/Circuit2.png" alt="" style="position: relative; top: 54px;" /></a>