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Team:Groningen/Notebook/Wetwork 13September2012
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(Created page with "{{HeaderGroningen2012}} <div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;"> Renske Measured FNR-GFP inside psac-cm inside B.subtilis 168. Same s...") |
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further microscopic part is executed by Arjan/Elbrich: no results. | further microscopic part is executed by Arjan/Elbrich: no results. | ||
| + | |||
| + | Elbrich | ||
| + | |||
| + | pSac-Cm + RFP NanoDrop | ||
| + | |||
| + | 1: 231,3 ng/ul | ||
| + | 2: 131,0 ng/ul | ||
| + | 3: 238,9 ng/ul | ||
| + | 4: 166,8 ng/ul | ||
| + | |||
| + | After cutting, sizes on gel were correct: 2 bands, one at 5000 bp (pSac-Cm) and one at 1000 bp (RFP.s | ||
Revision as of 14:57, 24 September 2012
Renske
Measured FNR-GFP inside psac-cm inside B.subtilis 168. Same setup as 12/09.
After setup OD =
- 0.889 (rotten meat, FNR-GFP)
- 1.040 (no meat, WT)
- 0.675 (no meat, FNR-GFP)
- 1.435 (no meat, FNR-GFP, not inoculated again (old culture to see wether stationary phase induces GFP))
further microscopic part is executed by Arjan/Elbrich: no results.
Elbrich
pSac-Cm + RFP NanoDrop
1: 231,3 ng/ul 2: 131,0 ng/ul 3: 238,9 ng/ul 4: 166,8 ng/ul
After cutting, sizes on gel were correct: 2 bands, one at 5000 bp (pSac-Cm) and one at 1000 bp (RFP.s
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