Team:Grenoble/Modeling/Amplification/ODE
From 2012.igem.org
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- | We don’t take into account in the equations the growth of the bacterium. Indeed, the bacterium grows as long as it has some glucose. However, as long as there is glucose the | + | We don’t take into account in the equations the growth of the bacterium. Indeed, the bacterium grows as long as it has some glucose. However, as long as there is glucose the protein AC (adenylate cyclase) is not active, thus no cAMP is produced. |
Indeed, the biologists in order to check the “AND gate” behavior, the biologists built, see protocol <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test">protocol "AND gate test"</a> . Here we give the biological graphs of the absorbance and the graph of the RFU in function of the time for arabinose and cAMP maximum: | Indeed, the biologists in order to check the “AND gate” behavior, the biologists built, see protocol <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test">protocol "AND gate test"</a> . Here we give the biological graphs of the absorbance and the graph of the RFU in function of the time for arabinose and cAMP maximum: | ||
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Revision as of 08:25, 21 September 2012
Preliminary
We will use the quasi steady state approximation (QSSA) then. The idea is that there are quick reactions, such as enzymatic ones, complexations, etc… And there are slow reactions such as protein production. We assume that the evolution speed of an element that is created only by quick reaction is null.Goal
In this part, we want to answer to three questions:- What is the sensitivity of our system?
- What is the time response?
- What steady states will our system always reach?