Team:Groningen/Notebook/Wetwork 21August2012

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Tom
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Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees celcius.
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Result: None of the combined promotors with GFP resulted in the correct size band.
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Tom<br>
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<br>
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Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees celcius. <br>
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<br>
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Result: None of the combined promotors with GFP resulted in the correct size band.<br>
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<br>
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Emeraldo<br>
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Cut AmilGFP, BBa_K274110, and pSac-Cm+terminator with EcoRI and PstI, run on the gel and excised the correct bands (the AmilGFP band was not detected). Ligation reaction for cut BBa_k274110 and cut pSac-CM+terminator. Transformation of E.coli DH5alpha with the ligated product.<br>
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<br><br>
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Arjan
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<br>
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GC-MS: blank runs of the three organic solvents used<br>
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<br>
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Nisa <br>
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PCR for checking the construct pigment <br>
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result: PCR result was ran in an agarose gel. The result showed multifragment DNA which can imply multi-annealing temperature for every PCR reaction. We cut the DNA fragment with the correct size and purified it.
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<br><br>
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Yonathan<br>
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Initiation of attempts to ligate Violacin with pFnr and pLacI
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 18:41, 26 September 2012







Tom

Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees celcius.

Result: None of the combined promotors with GFP resulted in the correct size band.

Emeraldo
Cut AmilGFP, BBa_K274110, and pSac-Cm+terminator with EcoRI and PstI, run on the gel and excised the correct bands (the AmilGFP band was not detected). Ligation reaction for cut BBa_k274110 and cut pSac-CM+terminator. Transformation of E.coli DH5alpha with the ligated product.


Arjan
GC-MS: blank runs of the three organic solvents used

Nisa
PCR for checking the construct pigment
result: PCR result was ran in an agarose gel. The result showed multifragment DNA which can imply multi-annealing temperature for every PCR reaction. We cut the DNA fragment with the correct size and purified it.

Yonathan
Initiation of attempts to ligate Violacin with pFnr and pLacI

Back to notebook

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