Team:Groningen/Notebook/Wetwork 11July2012

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AlsT PCR with Pfu and Taq polymerase at 60 °C with primers 150, 250, 300, 500 bp.
 
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Plasmid isolation of Biobrick parts
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AlsT PCR with Pfu and Taq polymerase at 60 °C with primers 150, 250, 300, 500 bp.<br>
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Nisa/Emeraldo<br>
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Plasmid isolation of Biobrick parts BBa_K116603, BBa_K116602, BBa_J31019, BBa_K116639, BBa_K116609. Concentrations of 69.9, 76.5, 109.8, 70.2, 106.2 ng/ul respectively.<br>
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EcoRI restiction of with these isolated plasmids.<br>
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Arjan/Renske<br>
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D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5.<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5.
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Latest revision as of 21:03, 25 September 2012







Tom/Emeraldo

AlsT PCR with Pfu and Taq polymerase at 60 °C with primers 150, 250, 300, 500 bp.

Nisa/Emeraldo

Plasmid isolation of Biobrick parts BBa_K116603, BBa_K116602, BBa_J31019, BBa_K116639, BBa_K116609. Concentrations of 69.9, 76.5, 109.8, 70.2, 106.2 ng/ul respectively.
EcoRI restiction of with these isolated plasmids.


Arjan/Renske

D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5.


Back to notebook



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