Team:Groningen/Notebook/Wetwork 3July2012

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Micro-array harvest experiment
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Experimental duplo of 02/07. Cells harvested and kept in -80.
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Micro-array harvest experiment (Arjan/Renske)<br><br>
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Nisa/Emeraldo
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Experimental duplo of 02/07. Cells harvested and kept in -80.<br><br>
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1.) Linearization of: BBa_K274100 (red pigment), BBa_K274002 (violacein-violet pigment), BBa_K090403 (amyE integration plasmid), BBa_R0062 (luxR promoter), BBa_J37034 (luxI, GFP added). All of them are in correct length except the integration plasmid (expected)
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Nisa/Emeraldo<br><br>
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1.) Linearization of: BBa_K274100 (red pigment), BBa_K274002 (violacein-violet pigment), BBa_K090403 (amyE integration plasmid), BBa_R0062 (luxR promoter), BBa_J37034 (luxI, GFP added). All of them are in correct length except the integration plasmid (expected)<br>
2.) B. subtilis transformation using BBa_K090403 and BBa_I742123 by Ruud from Molgen group (to compare with our work)
2.) B. subtilis transformation using BBa_K090403 and BBa_I742123 by Ruud from Molgen group (to compare with our work)
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Tom<br><br>
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Isolating chromosomal DNA from Basillus subtilis.<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 16:55, 25 September 2012









Micro-array harvest experiment (Arjan/Renske)

Experimental duplo of 02/07. Cells harvested and kept in -80.

Nisa/Emeraldo

1.) Linearization of: BBa_K274100 (red pigment), BBa_K274002 (violacein-violet pigment), BBa_K090403 (amyE integration plasmid), BBa_R0062 (luxR promoter), BBa_J37034 (luxI, GFP added). All of them are in correct length except the integration plasmid (expected)
2.) B. subtilis transformation using BBa_K090403 and BBa_I742123 by Ruud from Molgen group (to compare with our work)

Tom

Isolating chromosomal DNA from Basillus subtilis.

Back to notebook