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Team:Calgary/Notebook/Protocols/bacgrowth
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| - | + | <ol> | |
| + | <li>Autoclave the spinner flask with copper sparger in the correct position.</li> | ||
| + | <li>Mix and autoclave 1L LB solution and add to the spinner flask.</li> | ||
| + | <li>Add 1 ml ampicillin and 1ml ampicillin-resistant <i>E coli</i> into the LB media.</li> | ||
| + | <li>Put the spinner flask in the incubator at 37°C.</li> | ||
| + | <li>Remove 200µL for a sample and measure the optical density in reference to the optical density of a LB sample (negative control).</li> | ||
| + | <li>Repeat the experiment three times: one with the spinner only, one with the air sparger only, and one with both air sparger and spinner on.</li> | ||
| + | </ol> | ||
| + | <br><br><br><br><br><br><br><br> | ||
</html>}} | </html>}} | ||
Latest revision as of 07:23, 3 October 2012
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Bacterial Growth Experiment
- Autoclave the spinner flask with copper sparger in the correct position.
- Mix and autoclave 1L LB solution and add to the spinner flask.
- Add 1 ml ampicillin and 1ml ampicillin-resistant E coli into the LB media.
- Put the spinner flask in the incubator at 37°C.
- Remove 200µL for a sample and measure the optical density in reference to the optical density of a LB sample (negative control).
- Repeat the experiment three times: one with the spinner only, one with the air sparger only, and one with both air sparger and spinner on.
