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Team:Calgary/Notebook/Protocols/nucleaseassay
From 2012.igem.org
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| - | <li>E. coli genome was prepared using MP bio kit and the DNA was quantified using picogreen assay | + | <li>E. coli genome was prepared using MP bio kit and the DNA was quantified using <a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/picogreen">picogreen assay.</a> |
<li>2µg of DNA was put into each test condition. | <li>2µg of DNA was put into each test condition. | ||
<li>Appropriate buffer(s) were added for each enzyme/enzyme combination in the tubes. | <li>Appropriate buffer(s) were added for each enzyme/enzyme combination in the tubes. | ||
| - | <li>10 units of enzyme was added into appropriate conditions | + | <li>10 units of enzyme was added into appropriate conditions. |
<li>At each timepoint aliquots were taken out of the reaction tubes and run on a 1% gel for 40 minutes and imaged. | <li>At each timepoint aliquots were taken out of the reaction tubes and run on a 1% gel for 40 minutes and imaged. | ||
</ol> | </ol> | ||
| - | <br><br><br><br><br><br><br><br> | + | <br><br><br><br><br><br><br><br><br><br><br> |
</html>}} | </html>}} | ||
Latest revision as of 02:48, 4 October 2012
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Nuclease assay
- E. coli genome was prepared using MP bio kit and the DNA was quantified using picogreen assay.
- 2µg of DNA was put into each test condition.
- Appropriate buffer(s) were added for each enzyme/enzyme combination in the tubes.
- 10 units of enzyme was added into appropriate conditions.
- At each timepoint aliquots were taken out of the reaction tubes and run on a 1% gel for 40 minutes and imaged.
