Team:NYMU-Taipei/ymip2.html

From 2012.igem.org

(Difference between revisions)
(Created page with "<html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>NYMU iGEM</title> <link href="http://www.royals.co...")
 
(5 intermediate revisions not shown)
Line 64: Line 64:
   <div id="ymi_header">
   <div id="ymi_header">
       <div id="inner_header">
       <div id="inner_header">
-
       <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/7/7d/Ymi_header.jpg" border="0"></a>
+
       <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/1/15/Ymi_header1.jpg" border="0"></a>
       </div>
       </div>
   </div>
   </div>
Line 79: Line 79:
       <div id="ymi_left_column">
       <div id="ymi_left_column">
  <div class="text_area" align="justify">
  <div class="text_area" align="justify">
-
<div class="title">Protocol</div>
+
<div class="title">Mesenchymal Stem Cells Culturing</div>
<div align="left">
<div align="left">
-
  <p><span class="subtitle">Mesenchymal Stem Cells Culturing</span></p>
+
 
</div>
</div>
<div align="left">
<div align="left">
Line 116: Line 116:
culturing</a></li>
culturing</a></li>
                 <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells"  
                 <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells"  
-
href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic<br />
+
href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells</a></li>
-
Cells into Stem Cell &amp; <br />
+
-
Separation of iPS cells</a></li>
+
                 <li></li>
                 <li></li>
                 <li></li>
                 <li></li>
Line 133: Line 131:
                 <li><a title="Safety"   
                 <li><a title="Safety"   
href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li>
href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li>
-
                 <li><a title="Collaboration"   
+
                 <li><a title="Collaboration with NTU"  href="https://2012.igem.org/Team:NYMU-Taipei/ymico1.html">Collaboration with NTU</a></li>
-
href="https://2012.igem.org/Team:NYMU-Taipei/ymico.html">Collaboration</a></li>
+
 
 +
                <li><a title="NYMU Bioenergy Breakthrough"  href="https://2012.igem.org/Team:NYMU-Taipei/ymibt.html">NYMU Bioenergy<br />
 +
Breakthrough</a></li>
                 <li><a title="Parts"   
                 <li><a title="Parts"   
href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li>
href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li>

Latest revision as of 17:34, 26 October 2012

NYMU iGEM

Mesenchymal Stem Cells Culturing

  1. Mesenchymal stem cells from human umbilical cord were provided by Prof. Chun-Min Lo from National Yang Ming University.
  2. View cultures using a microscopy and after the cultures was full of the dish, remove the spent medium.
  3. Wash the cell monolayer with PBS and remove it.
  4. Add HyQTase(1ml per 25 cm2 of surface area) and incubate for 1 minutes.
  5. After making sure cells were detached, resuspend the cells with medium(more than 5 folds volume of HyQTase).
  6. Centrifuge under 2000 rpm, 5minutes, 25°C.
  7. Remove the supernatant and add 5 ml medium to suspend cells.
  8. Count the cells by Bright Line Counting Chamber. Subject the cell diversity to 104/ml
  9. Seed the cells for 104/well, 1ml/well in the 24-well plate.
  10. Until cells grow full of each well, do the coculturing experiment.

Retrieved from "http://2012.igem.org/Team:NYMU-Taipei/ymip2.html"