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Revision as of 14:01, 26 September 2012 (view source) Alicja (Talk | contribs) ← Older edit		Latest revision as of 14:08, 26 September 2012 (view source) Tomvanlente (Talk | contribs)
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-	Plasmid isolation of <br>	+	Plasmid isolation of sboA + Psaccm and fnr + Psaccm. Restriction analysis with EcoRI and PstI. S22, S23, S29, S210, S13 and S17 had the correct insert.<br>
	<br>		<br>
	Elbrich<br>		Elbrich<br>

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Latest revision as of 14:08, 26 September 2012

Tom

Plasmid isolation of sboA + Psaccm and fnr + Psaccm. Restriction analysis with EcoRI and PstI. S22, S23, S29, S210, S13 and S17 had the correct insert.

Elbrich

Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul.

To control the cutting sites of the Bricks, BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel.


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