Team:Groningen/Notebook/Wetwork 25June2012

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Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR))
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Tom <br>
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Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth. <br><br>
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Nisa and Emeraldo <br>
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Preparation for another B. subtilis transformation (BBa_K090403+pigment):<br>
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1.) Preparation of Transformation medium (SMM)<br>
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2.) O/N culture of B. subtilis in SMM<br>
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3.) O/N culture E. coli with BBa_K090403<br>
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<br>
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Primer design for P alsT with addition of prefix and suffix<br>
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1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene)<br>
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2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes<br>
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3.) Order the primers<br>
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<br>
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Preparing starch agar and iodine for checking purpose of B. subtilis transformant <br>
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(B. subtilis transformants of BBa_K090403 should not be able to degrade starch)<br>
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<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 16:50, 25 September 2012








Tom
Transformation of biobricks BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)) was succesfull and transferred to LB broth for overnight growth.

Nisa and Emeraldo
Preparation for another B. subtilis transformation (BBa_K090403+pigment):
1.) Preparation of Transformation medium (SMM)
2.) O/N culture of B. subtilis in SMM
3.) O/N culture E. coli with BBa_K090403

Primer design for P alsT with addition of prefix and suffix
1.) check promoter region (150bp, 250 bp, 300bp, and 500bp before the gene)
2.) add restriction enzyme sites and 5 bases for plateau of restriction enzymes
3.) Order the primers

Preparing starch agar and iodine for checking purpose of B. subtilis transformant
(B. subtilis transformants of BBa_K090403 should not be able to degrade starch)

Back to notebook



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