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Team:Grenoble/Project/Abstract
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huge impacts on our societies and ways diagnosis and prevention will be performed. | huge impacts on our societies and ways diagnosis and prevention will be performed. | ||
In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector. | In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector. | ||
| - | It consists of three interconnected modules: | + | <br/><br/>It consists of three interconnected modules: |
| + | <ol> | ||
| + | <li>Detection</li> | ||
| + | <li>Amplification/Communication</li> | ||
| + | <li>Output</li> | ||
| + | </ol> | ||
| + | <br/> | ||
The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false | The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false | ||
positive outputs. Once amplified and filtered, the signal is transmitted to neighboring | positive outputs. Once amplified and filtered, the signal is transmitted to neighboring | ||
bacteria via a diffusible molecule. In turn, the amplification loop is triggered which | bacteria via a diffusible molecule. In turn, the amplification loop is triggered which | ||
| - | leads to the production of a measurable fluorescence output. The design of our | + | leads to the production of a measurable fluorescence output. <br/> |
| + | <br/>The design of our | ||
network is easily adaptable to different input signals by using other receptor domains. | network is easily adaptable to different input signals by using other receptor domains. | ||
| - | <center> <h4>Scheme of the concept</h4> </center> | + | <!-- <center> <h4>Scheme of the concept</h4> </center> |
<center><img src="https://static.igem.org/mediawiki/2012/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/></center> | ||
<center> <h4>Theoretical response of the device depending on the input signal</h4> <br/> | <center> <h4>Theoretical response of the device depending on the input signal</h4> <br/> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/c/cf/Schema2.png" alt="Theoretical response of the device depending on the input signal" style="horizontal-align: center;"/></center> | + | <img src="https://static.igem.org/mediawiki/2012/c/cf/Schema2.png" alt="Theoretical response of the device depending on the input signal" style="horizontal-align: center;"/></center> --> |
</section> | </section> | ||
</div> | </div> | ||
Latest revision as of 00:59, 25 September 2012
sEnsiColi: A tunable and reliable ultra-sensitive detector
Multi-resistant bacteria are a worldwide issue which in a very near future will have huge impacts on our societies and ways diagnosis and prevention will be performed. In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.It consists of three interconnected modules:
- Detection
- Amplification/Communication
- Output
The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false positive outputs. Once amplified and filtered, the signal is transmitted to neighboring bacteria via a diffusible molecule. In turn, the amplification loop is triggered which leads to the production of a measurable fluorescence output.
The design of our network is easily adaptable to different input signals by using other receptor domains.
