Team:Groningen/Notebook/Wetwork 13September2012

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Renske
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Measured FNR-GFP inside psac-cm inside B.subtilis 168. Same setup as 12/09.
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After setup OD =
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*0.889 (rotten meat, FNR-GFP)
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*1.040 (no meat, WT)
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*0.675 (no meat, FNR-GFP)
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*1.435 (no meat, FNR-GFP, not inoculated again (old culture to see wether stationary phase induces GFP))
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further microscopic part is executed by Arjan/Elbrich: no results.
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Elbrich
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Renske<br>
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<br>
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Measured FNR-GFP inside psac-cm inside B.subtilis 168. Same setup as 12/09.<br>
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<br>
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After setup OD =
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<ul>
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<li>0.889 (rotten meat, FNR-GFP)</li>
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<li>1.040 (no meat, WT)</li>
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<li>0.675 (no meat, FNR-GFP)</li>
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<li>1.435 (no meat, FNR-GFP, not inoculated again (old culture to see wether stationary phase induces GFP))
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</ul>
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<p class="margin">further microscopic part is executed by Arjan/Elbrich: no results.
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<br><br>
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Elbrich<br>
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<br>
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pSac-Cm + RFP NanoDrop<br>
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<br>
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1: 231,3 ng/ul<br>
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2: 131,0 ng/ul<br>
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3: 238,9 ng/ul<br>
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4: 166,8 ng/ul<br>
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<br>
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After cutting, sizes on gel were correct: 2 bands, one at 5000 bp (pSac-Cm) and one at 1000 bp (RFP)<br> <br>
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Nisa <br> <br>
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Result of E. coli transformation: we FINALLY have some blue colonies! E. coli containing fnr+amilCP and sboA+amilCP appeared blue. The other transformans are white/yellowish and white/pinkish(not very sure about the colour differences)<br><br>
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O/N culture all the transformans for further checking!
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pSac-Cm + RFP NanoDrop
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<br>
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<br>
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1: 231,3 ng/ul
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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2: 131,0 ng/ul
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</p><br><br>
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3: 238,9 ng/ul
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</body>
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4: 166,8 ng/ul
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After cutting, sizes on gel were correct: 2 bands, one at 5000 bp (pSac-Cm) and one at 1000 bp (RFP)
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<html>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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</html>
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Latest revision as of 15:04, 26 September 2012







Renske

Measured FNR-GFP inside psac-cm inside B.subtilis 168. Same setup as 12/09.

After setup OD =

  • 0.889 (rotten meat, FNR-GFP)
  • 1.040 (no meat, WT)
  • 0.675 (no meat, FNR-GFP)
  • 1.435 (no meat, FNR-GFP, not inoculated again (old culture to see wether stationary phase induces GFP))

further microscopic part is executed by Arjan/Elbrich: no results.

Elbrich

pSac-Cm + RFP NanoDrop

1: 231,3 ng/ul
2: 131,0 ng/ul
3: 238,9 ng/ul
4: 166,8 ng/ul

After cutting, sizes on gel were correct: 2 bands, one at 5000 bp (pSac-Cm) and one at 1000 bp (RFP)

Nisa

Result of E. coli transformation: we FINALLY have some blue colonies! E. coli containing fnr+amilCP and sboA+amilCP appeared blue. The other transformans are white/yellowish and white/pinkish(not very sure about the colour differences)

O/N culture all the transformans for further checking!

Back to notebook



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